What is Glucoamylase Powermix 2.0?
The product is produced by mixing carefully optimized proportion of glucoamylase and pullulanase coming from submerged fermentation of Aspergillus niger and Bacillus subtilis, respectively. The combination of glucoamylase with supplementary pullulanase makes saccharification more efficient by avoiding limit dextrin and by-product formation, and therefore saving processing cost while producing more glucose.
Glucoamylase Powermix 2.0 Product Characteristics
Declared Activity
Glucoamylase 90,000 u/ml
Pullulanase 1,000 u/ml
Production Organism
Aspergillus niger
Bacillus subtilis
Physical Form
Liquid
Color
Brown
Color may vary from batch to batch. Color intensity is not an indication of enzyme activity.
Odour
Normal microbial fermentation odour.
Production Method
Produced by submerged fermentation, separation and extraction technology.
Definition of Unit
1 unit of Glucoamylase equals to the amount of enzyme which hydrolyzes soluble starch to get 1mg glucose at 40℃ and pH4.6 in 1h.
1 unit of Pullulanase equals to the amount of enzyme needed to liberate 1µmol of reducing sugar (Calculated as glucose) from pullulan in one minute at pH5.8, 50℃.
Glucoamylase Powermix 2.0 Product Specification
Lower Limit
Upper Limit
Enzyme activity
Glucoamylase 90,000 u/ml
Pullulanase 1,000 u/ml
Lead
5 mg/kg
Arsenic
3 mg/kg
Total viable count
50,000 CFU/ml
Coliform Bacteria
30 CFU/ml
Escherichia coli
10 CFU/ml
3 MPN/ml
Salmonella
Not Detected/25ml
Glucoamylase Powermix 2.0 Reaction Parameters
Activity Temperature
30℃-65℃
Optimum Temperature
58℃-64℃
Activity pH
3.5-6.0
Optimum pH
4.0-4.8
Glucoamylase Powermix 2.0 Benefits
Efficient degradation of dextrins
• Higher dextrose yield (DX＞96%) and Less by-products formation
• Low treatment costs and dosages
Glucoamylase Powermix 2.0 Dosage
The recommended dosage is 0.35-0.6 kg of the enzyme preparation per ton of dry starch when saccharifying at 60-63℃ and pH 4.3-4.5. A starting dose of 0.45kg per ton of dry starch is recommended under most conditions. However, the optimal dosage depends on the specific process parameters and should be determined by testing different dosages.
Glucoamylase Powermix 2.0 Recommended Storage
Best Before
When stored as recommended, the product is best used within 6 months from date of delivery
Shelf Life
6 months at 25℃, activity remains≥90%; Increase dosage after shelf life.
Storage Conditions
This product should be stored in a cool and dry place in sealed container, avoiding insolation, high temperature and damp. The product has been formulated for optimal stability. Extended storage or adverse conditions such as higher temperature or higher humidity may lead to a higher dosage requirement.
Glucoamylase Powermix 2.0 Packaging
25kgs/drum; 1,125kgs/drum.
Safety and Handling Precautions
Enzyme preparations are proteins, which may induce sensitization and cause allergic type reactions in sensitized individuals. Prolonged contact may cause minor irritation for skin, eyes or nasal mucosa, so any direct contiguity with human body should be avoided. If irritation or allergic response for skin or eyes develops, consult a doctor.
