Description
Tris Base
Tris Base Quick Details
Chemical Name: Tris Base
CAS No.: 77-86-1
Molecular Fomula: C4H11NO3
Chemical Structure:
Tris Base Molecular Weight: 121.14
Tris Base PH(5%):10.0-11.5
Appearance: White crystal powder
Assay：99.5%
Tris Base Typical Properties
Item
Specifications
Results
Content
99.5%—101.0%
100.34%
Moisture（by karl fischer）
≤0.3%
0.19%
Insolubles
≤0.005%
0.00%
PH(5%)
10.0-11.5
10.65
UV Absorbance（290nm,40%）
≤0.2
0.050
UV Absorbance（400nm 40%）
≤0.2
0.011
Heavy metals（as pb）
≤5ppm
＜5ppm
Fe
≤5ppm
＜5ppm
Cl
≤3ppm
＜5ppm
Solubility （1.0M solution in water）
Immediately soluble yielding a clear haze-free solution free of particulates
Residue on ignition
≤0.05%
0.00%
Melting point（℃）
168.0-172.0
171.5-171.9
Appearance
White crystal powder
Tris Base Application
1. Tris is often used as a biological buffer with a pH of 6.8, 7.4, 8.0, 8.8. Its structural formulaandpH value varygreatly with temperature. In general, for every degree of temperature increase, the pH drops by 0.03.
2. Tris is widely used in the preparation of buffers for biochemical and molecular biology experiments. For example, tries are commonly used in TAE and TBE buffers (for the solubilization of nucleic acids) andin biochemical experiments. Since it contains an amino group, it can undergo a condensation reaction with an aldehyde.
3. Tris is a weak base, and its pKa is 8.1 at room temperature (at 25 ° C; according to the buffer theory, the effective buffer range of Tris buffer is between pH 7.0 and 9.2.
4. The pH of the aqueous solution of the Tris base is about 10.5. Usually, hydrochloric acid is added to adjust the pH to the desired value to obtain a buffer of the pH. But at the same time, attention should be paid to the effect of temperature on the pKa of Tris.
Tris Base Packaging and Shipping
Tris Base25kgs per drum
Tris Base Storage
Dry cool place
